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Xylose Lysine Deoxycholate (XLD) Agar TM 492-100gm | Growth medium used for the isolation of Salmonella and Shigella species from clinical samples and from food

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About the item

About this item XLD Agar is a selective differential medium for the isolation of Gram-negative enteric pathogens from fecal specimens and other clinical material. It is especially suitable for the isolation of Shigella and Salmonella species. Microbiological testing of foods, water and dairy products. Dissolve 56.68g in 1000ml distilled water. Cool to 45 - 50 ̊C prior to dispense into sterile petri plates.

PRODUCT SUMMARY AND EXPLANATION: XLD Agar was formulated by Taylor for the isolation and differentiation of enteric pathogens including Salmonella typhi from other Salmonella species. XLD Agar has been recommended for the identification of Enterobacteriaceae and for the microbiological testing of foods, water and dairy products. The media formulation does not allow the over growth of other organisms over Salmonella and Shigella. XLD Agar is one of the media used in the Microbial Limit Tests in the USP & EP PRINCIPLE: The medium contains Yeast extracts as source of s and minerals. Addition of Sodium deoxycholate acts as a selective agent which is inhibitory to Gram-positive bacteria. It suppresses the growth of other enteric pathogens and enhances the growth of only few enteric bacilli. The medium contains Xylose as a fermentable carbohydrate which is utilized by Salmonella species. The medium pH is changed due to fermentation of Xylose which is detected by indicator Phenol red, thus the colony colour turns red. The medium also contains Lactose and Sucrose as the source of fermentable sugar. L-lysine is an essential amino source. Lysine is added to differentiate Salmonella Sp. Sodium chloride helps maintaining the osmotic balance of the cells. This medium also allows differentiation of bacilli based on their ability to produce H2S; it contains Sodium thiosulphate and Ferric ammonium citrate that helps visualizing the black centered colonies on production of hydrogen sulphide in the medium. Agar is added as the solidifying agent.

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